Plasmid_Backbone

Part:BBa_J428388:Design

Designed by: Marcos Valenzuela-Ortega   Group: 2021 Engineering Committee   (2022-06-16)


pJUMP19-min2 Level 0 Minimal cargo vector.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3030
    Illegal suffix found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3030
    Illegal SpeI site found at 6
    Illegal PstI site found at 20
    Illegal NotI site found at 13
    Illegal NotI site found at 3036
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3030
    Illegal suffix found at 6
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3030
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3045
    Illegal SpeI site found at 6
    Illegal PstI site found at 20
    Illegal NgoMIV site found at 857
    Illegal NgoMIV site found at 986
    Illegal NgoMIV site found at 1480
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2278


Design Notes

Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003


Source

Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

References